The surface you select for your precoated cultureware affects cell morphology, phenotype, and function, so it's essential that you get it right for upstream and downstream cell culture applications. By using the optimal cell type surface in your research, you can control variables in experimental data, more closely mimicin vivoconditions, promote cell growth, and achieve the desired endpoints.
然而,表面的选择并不总是straightforward. The options seem endless, and many have overlapping characteristics. It can be hard to determine what works best for your needs. This guide can help.
3 Surface Categories to Know
When selecting the right cultureware for your work, it's important to understand the three extracellular matrix (ECM) proteins used as coatings: natural, mimetic, and synthetic.
- Natural coatingsincludeECMs and biologically coated surfaces, which help scientists mimicin vivoenvironments for 2D and 3D cultures. Options includecollagen,laminin, or surfaces such as康宁®Matrigel®Matrix.
- Mimetic coatingsincludeECM mimetic and advanced surfaces, which can support specialized cell expansion and assay applications via unique functional surface activity. Mostly applied in cell therapies, mimetic coatings use a small portion of the synthesized protein to support binding.
- Synthetic coatingsinclude enhanced tissue surfaces that modify cultureware with positive, negative, or positive-negative charges, depending on cell type. Synthetic coatings can promote the attachment and growth of fastidious cell types, including primary or transfected cell lines in low- or serum-free environments.